The group provides expertise and bioinformatics analysis tools to LRI scientists. Areas of focus for the group include next generation sequencing, functional genomics, microarray data analysis, biological sequence analysis, genome analysis and biostatistics.
The group has advanced skills in bioinformatics, computing & programming, statistics and molecular biology enabling us to provide world class bioinformatics support to CRUK's research objectives.
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Solexa runs generating more than 50 million reads per run. |
We are currently analysing data in the following application areas
- ChIP-Seq The sequencing of protein bound DNA fragments.
- RNA-Seq The sequencing of RNA to determine sequence and abundance.
- Resequencing Genomic scale resequencing to identify genomic variants.
- miRNA The characterisation and quantification of small regulatory RNA moleclues.
- 4C, 5C, 6C
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We offer a comprehensive microarray analysis service for expression, CGH, tiling, SNP and methylation arrays. |
Microarray services
- Experimental Design Getting the most out of your experiment.
- Expression Identifying condition dependent mRNA changes.
- CGH: Finding regions of genomic loss and gain across samples.
- Tiling Arrays Localising protein-DNA interations via ChIP-chip.
- SNP Arrays Determining genotype, copy number variation and loss of heterozygosity.
Tools and techniques
- Pathway Analysis Identifying functional signatures within your list of genes.
- GeneGo Pathway analysis software
- Transcription Factor Analysis Identifying putative binding motifs within target promoter sequences.
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Gene and protein level analysis using genome, sequence and annotation databases. |
Problems we solve
- Searching for novel homologues/orthologues.
- Protein characterisation.
- Promoter analysis.
- Help with the genome browsers.
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We also have the following skills within the group that may be of use in your research. |
Skills and expertise
- Data visualisation and the production of publication quality figures.
- Web development and the deployment of tools and data over the internet.
- Computer programming expertise in R, Perl, C, Java, Python, PHP, Bash.
- Database programming and the storage of complex data.
Statistical advice
- Experimental design.
- The appropriate statistical method to apply.
- Advice on specialist software.
- Statistical analysis of data.
- Analysis of RNAi screens
